Verify the IIS Worker Process User Permissions

Typically, .NET web applications run in an IIS worker process. By default, the IIS worker process name is w3wp.exe or aspnet_wp.exe. The default account that runs the worker process is NETWORK SERVICE. When you install the .NET agent, the installer creates the root installation directory. The root directory is given the appropriate permissions that allow access to the .NET <Agent_Home> directory. By default, the .NET <Agent_Home> directory is named the wily directory. The installer also creates the bin, ext, and log subdirectories and the Performance Monitor counters.
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Typically, .NET web applications run in an IIS worker process. By default, the IIS worker process name is w3wp.exe or aspnet_wp.exe. The default account that runs the worker process is 
NETWORK SERVICE
. When you install the .NET agent, the installer creates the root installation directory. The root directory is given the appropriate permissions that allow access to the .NET 
<Agent_Home>
 directory. By default, the .NET 
<Agent_Home>
 directory is named the 
wily
 directory. The installer also creates the bin, ext, and log subdirectories and the Performance Monitor counters.
You can, however, configure other accounts to run the IIS worker process at the application pool level. 
Follow these steps: